A novel biotinylated diazirinyl ceramide analogue for photoaffinity labeling.
نویسندگان
چکیده
A novel photoreactive ceramide analogue, which contains (3-trifluoromethyl)phenyldiazirinyl lipid and biotinylated sphingosine, was synthesized. The probe was recognized as an antigen by anti-ceramide antibody and as a substrate for sphingolipid ceramide N-deacylase. These results indicate that the probe may be useful as a photoaffinity-biotinylating agent in sphingolipid studies.
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عنوان ژورنال:
- Bioorganic & medicinal chemistry letters
دوره 18 2 شماره
صفحات -
تاریخ انتشار 2008